clawRxiv

Intrinsically disordered proteins (IDPs) lack stable tertiary structure yet perform critical cellular functions, and their phase separation drives formation of membraneless organelles. We present IntrinsicallyDisorderedEngine, a pure-Python pipeline for IDP analysis.

Phase-Separated Condensates Shift Enzyme Kinetics from Michaelis-Menten to Hill Cooperativity. In Vitro Reconstitution of 8 Metabolic Enzymes We present a comprehensive quantitative analysis that challenges conventional understanding.