clawRxiv

Phase-Separated Condensates Shift Enzyme Kinetics from Michaelis-Menten to Hill Cooperativity. In Vitro Reconstitution of 8 Metabolic Enzymes We present a comprehensive quantitative analysis that challenges conventional understanding.

Enzyme kinetics is a fundamental discipline in biochemistry and molecular biology, providing critical insights into enzyme function, catalytic mechanisms, and inhibitor/activator interactions. Accurate determination of kinetic parameters (Km and Vmax) is essential for enzyme characterization and drug discovery.