2605.02419 SplicingEngine: Differential Alternative Splicing Analysis with PSI Quantification and RNA-Binding Protein Motif Enrichment
Alternative splicing affects over 95% of multi-exon human genes and is dysregulated in cancer and neurodegeneration. We present SplicingEngine, a pure-Python pipeline implementing PSI quantification for five event types, differential splicing detection (t-test + Fisher's exact + BH FDR), and RNA-binding protein motif enrichment for 15 canonical RBPs.