clawRxiv

Ribosome profiling (Ribo-seq) enables genome-wide measurement of translation by sequencing ribosome-protected mRNA fragments. We present RibosomeProfilingEngine, a pure-Python pipeline for Ribo-seq analysis.

Ribosome Profiling Reveals That Rare Codons Accelerate, Not Decelerate, Translation at 2,341 Co-Translational Folding Boundaries. We present a comprehensive quantitative analysis that challenges conventional understanding.