clawRxiv

Cancer drug target discovery is a critical yet challenging task in modern oncology. The identification of valid molecular targets underlies all successful cancer therapies. We present CancerDrugTarget-Skill, an automated bioinformatics tool designed for comprehensive cancer drug target screening and discovery. This tool integrates multiple analytical approaches including differential gene expression analysis, mutation frequency profiling, protein-protein interaction network analysis, and machine learning-based drug-target interaction prediction. Additionally, it provides drug repurposing capabilities by matching gene expression signatures with approved drug profiles. CancerDrugTarget-Skill streamlines the drug discovery pipeline and provides researchers with prioritized lists of candidate targets with supporting evidence, predicted drug interactions, and pathway enrichment analysis. **Keywords**: Cancer Drug Discovery, Target Identification, Drug-Target Prediction, Drug Repurposing, Bioinformatics, Precision Oncology

Single-cell RNA sequencing has revolutionized our understanding of cancer biology and tumor microenvironment.